Usage:
fastq-dump [options] [ -A ] <accession>
fastq-dump [options] <path>
INPUT
-A|--accession <accession> Replaces accession derived from <path> in filename(s) and deflines
--table <table-name> (New) Table name within SRA format, default is SEQUENCE
PROCESSING
Read Splitting Sequence data may be used as raw or split into individual reads
--split-spot Split spots into individual reads
Full Spot Filters Applied to the full spot independently of --split-spot
-N|--minSpotId <rowid> Minimum spot id
-X|--maxSpotId <rowid> Maximum spot id
--spot-groups <[list]> Filter by SPOT_GROUP (member): name[,...]
-W|--clip Apply left and right clips
Common Filters Applied to spots when --split-spot is not set, otherwise - to individual reads
-M|--minReadLen <len> Filter by sequence length >= <len>
-R|--read-filter <[filter]> Split into files by READ_FILTER value
optionally filter by a value: pass|reject|criteria|redacted
-E|--qual-filter Filter used in early 1K Genomes data:
no sequences starting or ending with >= 10N
Filters for individual reads Applied only with --split-spot set
--skip-technical Dump only biological reads
OUTPUT
-O|--outdir <path> Output directory, default is '.'
Multiple File Options Setting this options will produce more than 1 file, which will be suffixed by splitting criteria.
--split-files Dump each read into a separate file.Files will received suffix corresponding to read number
--split-3 Legacy 3-file splitting for mate-pairs:
First 2 biological reads satisfying dumping conditions
are placed in files *_1.fastq and *_2.fastq
If only 1 biological read is dumpable - it is placed in *.fastq
Biological reads 3 and above are ignored.
-G|--spot-group Split into files by SPOT_GROUP (member name)
-R|--read-filter <[filter]> Split into files by READ_FILTER value
optionally filter by a value: pass|reject|criteria|redacted
-T|--group-in-dirs Split into subdirectories instead of files
-K|--keep-empty-files Do not delete empty files
FORMATTING
Sequence
-C|--dumpcs <[cskey]> Formats sequence in color space (default for SOLiD),cskey may be specified for translation
-B|--dumpbase Formats sequence in base sequence (default for other than SOLiD).
Quality
-Q|--offset <integer> Offset to use for quality conversion, default is 33
--fasta Fasta only, no qualities
Defline
-F|--origfmt Defline contains only original sequence name
-I|--readids Append read id after spot id as 'accession.spot.readid' on defline
--helicos Helicos style defline
--defline-seq <fmt> Defline format specification for sequence.
--defline-qual <fmt> Defline format specification for quailty.
<fmt> is string of characters and/or variables. Variables could be are one of:
$ac - accession, $si - spot id, $sn - spot name, $sg - spot group (barcode),
$sl - spot length in bases, $ri - read number, $rn - read name, $rl - read length in bases.
'[]' could be used for an optional output: if all vars in [] yield empty values whole group is not printed.
Empty value is empty string or 0 for numeric variables.
Ex: @$sn[_$rn]/$ri - '_$rn' is omitted if name is empty
OTHER:
-h|--help Output a brief explantion for the program
-V|--version Display the version of the program then quit
-L|--log-level <level> Logging level as number or enum string
One of (fatal|sys|int|err|warn|info) or (0-5)
Current/default is warn
-v|--verbose Increase the verbosity level of the program
Use multiple times for more verbosity